Measurement

Part:BBa_J100251:Design

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2016-03-16)


pClone Cherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1095
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1095
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1095
    Illegal BamHI site found at 948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1095
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1095
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 837
    Illegal BsaI.rc site found at 760


Design Notes

Made sure no Bsa I sites in mCherry sequence.


Source

J119137 came from the Registry. mCherry was synthesized.

References